Reconstitution of an Actin Cortex Inside a Liposome
نویسندگان
چکیده
منابع مشابه
Reconstitution of an actin cortex inside a liposome.
The composite and versatile structure of the cytoskeleton confers complex mechanical properties on cells. Actin filaments sustain the cell membrane and their dynamics insure cell shape changes. For example, the lamellipodium moves by actin polymerization, a mechanism that has been studied using simplified experimental systems. Much less is known about the actin cortex, a shell-like structure un...
متن کاملLiposome reconstitution of a minimal protein-mediated membrane fusion machine.
Biological membrane fusion is dependent on protein catalysts to mediate localized restructuring of lipid bilayers. A central theme in current models of protein-mediated membrane fusion involves the sequential refolding of complex homomeric or heteromeric protein fusion machines. The structural features of a new family of fusion-associated small transmembrane (FAST) proteins appear incompatible ...
متن کاملReassembly of contractile actin cortex
The contractile cortex is a 50-nm–2-μm-thick layer of cytoskeleton under the plasma membrane that is rich in actin fi laments, myosin II, and actin-binding proteins (Bray and White, 1988). Assembly dynamics and contractility of this layer are thought to generate cortical tension, drive cytokinesis, and play a central role in cell locomotion and tissue morphogenesis (Bray and White, 1988; Albert...
متن کاملReconstitution of Sand Specimens Using a Rainer System
Air pluviation is a method of preparing laboratory models to carry out many geotechnical experiments. In this study, a new air pluviation device has been developed by the researchers. For laboratory modeling, it is important to calibrate the device using air pluviation to produce accurate specimens with desirable conditions and relative densities. A series of laboratory tests were conducted to ...
متن کاملReconstitution and Protein Composition Analysis of Endocytic Actin Patches
BACKGROUND Clathrin-actin-mediated endocytosis in yeast involves the progressive assembly of at least 60 different proteins at cortical sites. More than half of these proteins are involved in the assembly of a branched network of actin filaments to provide the forces required for plasma membrane invagination. RESULTS To gain insights into the regulation of endocytic actin patch dynamics, we d...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2009
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2008.09.029